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Article Dans Une Revue Cytotechnology Année : 2013

Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis

Diane Dall'Osso
  • Fonction : Auteur
Nathalie Joli
  • Fonction : Auteur
Juliette Olivre
  • Fonction : Auteur
Fabrice Priouzeau
  • Fonction : Auteur
Thamilla Zamoum
  • Fonction : Auteur
Paola Furla
  • Fonction : Auteur

Résumé

The temperate symbiotic sea anemone Anemonia viridis, a member of the Cnidaria phylum, is a relevant experimental model to investigate the molecular and cellular events involved in the preservation or in the rupture of the symbiosis between the animal cells and their symbiotic microalgae, commonly named zooxanthellae. In order to increase research tools for this model, we developed a primary culture from A. viridis animal cells. By adapting enzymatic dissociation protocols, we isolated animal host cells from a whole tentacle in regeneration state. Each plating resulted in a heterogeneous primary culture consisted of free zooxanthellae and many regular, small rounded and adherent cells (of 3-5 mu m diameter). Molecular analyses conducted on primary cultures, maintained for 2 weeks, confirmed a specific signature of A. viridis cells. Further serial dilutions and micromanipulation allowed us to obtain homogenous primary cultures of the small rounded cells, corresponding to A. viridis ``epithelial-like cells''. The maintenance and the propagation over a 4 weeks period of primary cells provide, for in vitro cnidarian studies, a preliminary step for further investigations on cnidarian cellular pathways notably in regard to symbiosis interactions.

Dates et versions

hal-01546173 , version 1 (23-06-2017)

Identifiants

Citer

Stephanie Barnay-Verdier, Diane Dall'Osso, Nathalie Joli, Juliette Olivre, Fabrice Priouzeau, et al.. Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis. Cytotechnology, 2013, 65 (5), pp.697-704. ⟨10.1007/s10616-013-9566-2⟩. ⟨hal-01546173⟩
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