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Article Dans Une Revue Macromolecular Bioscience Année : 2011

Polyelectrolyte Multilayer Nanofilms Used as Thin Materials for Cell Mechano-Sensitivity Studies

T. Boudou
T. Crouzier
  • Fonction : Auteur
C. Nicolas
  • Fonction : Auteur
K. Ren
  • Fonction : Auteur
C. Picart
  • Fonction : Auteur

Résumé

Three types of multilayer films made from poly(L-lysine)/hyaluronan, chitosan/hyaluronan, and poly(allylamine hydrochloride)/poly(L-glutamic acid), were used to investigate the interplay between film mechano-chemical properties and cell adhesion. We showed that C2C12 myoblast adhesion and proliferation depended on the extent of film cross-linking for all films whatever their internal chemistry. Cell spreading areas were found to correlate with the film's stiffness and to be distributed over a unique curve. Immuno-staining of the cytoskeletal components revealed the formation of F-actin stress fibers and vinculin plaques only on stiff films. Finally, we compared our results with previous studies performed on polyacrylamide and PDMS gels, two recognized materials for mechano-sensitivity studies. We found that the effect of substrate stiffness on cell spreading is material-dependent.

Dates et versions

hal-00670224 , version 1 (14-02-2012)

Identifiants

Citer

T. Boudou, T. Crouzier, C. Nicolas, K. Ren, C. Picart. Polyelectrolyte Multilayer Nanofilms Used as Thin Materials for Cell Mechano-Sensitivity Studies. Macromolecular Bioscience, 2011, 11 (1), pp.77-89. ⟨10.1002/mabi.201000301⟩. ⟨hal-00670224⟩
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