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Article Dans Une Revue Lab on a Chip Année : 2011

Diamagnetically trapped arrays of living cells above micromagnets

Résumé

Cell arrays are of foremost importance for many applications in pharmaceutical research or fundamental biology. Although arraying techniques have been widely investigated for adherent cells, organization of cells in suspension has been rarely considered. The arraying of non-adherent cells using the diamagnetic repulsive force is presented. A planar arrangement of Jurkat cells is achieved at the microscale above high quality microfabricated permanent magnets with remanent magnetization of J(r) approximate to 1 T, in the presence of a paramagnetic contrast agent. The cytotoxicity of three Gd based contrast agents, Gd-DOTA, Gd-BOPTA and Gd-HP-DO3A, is studied. Among them, Gd-HP-DO3A appears to be the most biocompatible toward Jurkat cells. In close agreement with analytical simulations, diamagnetically 'suspended' cells have been successfully arrayed above square and honeycomb-like micromagnet arrays, which act as a "diamagnetophobic" surface. Living cell trapping is achieved in a simple manner using concentrations of Gd-HP-DO3A as low as 1.5 mM.
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Dates et versions

hal-01067514 , version 1 (24-10-2017)

Identifiants

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Paul Kauffmann, Ammara Ith, Daniel O'Brien, Victor Gaude, Florian Boué, et al.. Diamagnetically trapped arrays of living cells above micromagnets. Lab on a Chip, 2011, 11 (18), pp.3153-3161. ⟨10.1039/c1lc20232d⟩. ⟨hal-01067514⟩
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