Targeting of MuLV Gag to the plasma membrane is mediated by PI(4,5)P2 and PhosphatidylSerine - FMNT - Fédération Micro- et Nano- Technologies Accéder directement au contenu
Article Dans Une Revue Retrovirology Année : 2009

Targeting of MuLV Gag to the plasma membrane is mediated by PI(4,5)P2 and PhosphatidylSerine

Résumé

Membrane targeting by the modern human immunodeficiency viruses is dependent on the plasma membrane-located phospholipid PI(4,5)P2. In order to determine if evolutionarily distant retroviruses are targeted by a similar mechanism, we generated mutant Gag constructs in the matrix (MA) domain of the Murine Leukemia Virus (MuLV) and examined their binding to membrane models and phenotypes in cell culture. Mutations in the MA polybasic region altered Gag localization, membrane binding and virion production. In addition, we show that MA binds with good affinity to all the phosphatidylinositol phosphates but displays a strong specificity for PI(4,5)P2 only if enhanced by phophatidylserine. Virus production was strongly impaired by PI(4,5)P2 depletion under 5ptaseIV overexpression. Our results suggest that the N-terminal polybasic region of MA is essential for Gag targeting to the plasma membrane and Gag cellular trafficking. The binding of the MA domain to PI(4,5)P2 appears to be a conserved feature among retroviruses, despite the fact that the MuLV-MA domain is structurally different from that of HIV-1 and -2 and lacks a readily identifiable PI(4,5)P2 binding cleft.
Fichier principal
Vignette du fichier
1742-4690-6-S2-O15.pdf (129.74 Ko) Télécharger le fichier
1742-4690-6-S2-O15.xml (5.6 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Format : Autre

Dates et versions

inserm-00649349 , version 1 (07-12-2011)

Identifiants

Citer

Elise Hamard-Peron, Franceline Juilliard, Js Saad, C Roy, Philippe Roingeard, et al.. Targeting of MuLV Gag to the plasma membrane is mediated by PI(4,5)P2 and PhosphatidylSerine. Retrovirology, 2009, 6 (Suppl 2), 6 (Suppl 2):O15. ⟨10.1186/1742-4690-6-S2-O15⟩. ⟨inserm-00649349⟩
215 Consultations
256 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More