Single conical track-etched nanopore for a free-label detection of OSCS contaminants in heparin

Tianji Ma (1) , Emmanuel Balanzat (2) , Jean-Marc Janot (1) , Sébastien Balme (1)

1 IEM - Institut Européen des membranes
2 CIMAP - UMR 6252 - Centre de recherche sur les Ions, les MAtériaux et la Photonique

The heparin contamination by oversulfated chondroitin (OSCS) was at the origin of one major sanitary problem of last decade. Here we propose a novel strategy to detect OSCS from heparin solution based on conical nanopore functionalized with poly-L-lysine deposition to ensure its re-usability. This sensor is an excellent to detect low heparin concentration (from 25 ng/ml to 3 mu g/ml) using the modification of ionic current rectification. It also allows following the kinetic of heparin degradation by heparinase with a good correlation with results obtained by classical methods. The sensor is sensitive to the inhibition of heparinase by OSCS until a concentration of 200 pg/l representing 0.01% in weight in a heparin. This resolution is one order of magnitude lower than the one obtained by chromatography. For the first time, it was reached without fluorescence labeling

Mots clés: Nanopore; Heparin; OSCS; Track-etched

Domaines: Chimie

Tianji Ma, Emmanuel Balanzat, Jean-Marc Janot, Sébastien Balme. Single conical track-etched nanopore for a free-label detection of OSCS contaminants in heparin. Biosensors and Bioelectronics, 2019, 137, pp.207-212. ⟨10.1016/j.bios.2019.05.005⟩. ⟨hal-02184411⟩