Synthesis of Fibrin-Type I Collagen Biomaterials via an Acidic Gel

Kun Wang (1) , Marie Camman (1) , Gervaise Mosser (1) , Bernard Haye (1) , Léa Trichet (1) , Thibaud Coradin (1)

1 LCMCP-MATBIO - Matériaux et Biologie

Fibrin-Type I collagen composite gels have been widely studied as biomaterials, in which both networks are usually formed simultaneously at a neutral pH. Here, we describe a new protocol in which mixed concentrated solutions of collagen and fibrinogen were first incubated at acidic pH to induce fibrinogen gel formation, followed by a pH change to neutral inducing collagen fiber formation. Thrombin was then added to form fibrin-collagen networks. Using this protocol, mixed gels containing 20 mg.mL−1 fibrin and up to 10 mg.mL−1 collagen could be prepared. Macroscopic observations evidenced that increasing the content of collagen increases the turbidity of the gels and decreases their shrinkage during the fibrinogen-to-fibrin conversion. The presence of collagen had a minor influence on the rheological properties of the gels. Electron microscopy allowed for observation of collagen fibers within the fibrin network. 2D cultures of C2C12 myoblasts on mixed gels revealed that the presence of collagen favors proliferation and local alignment of the cells. However, it interferes with cell differentiation and myotube formation, suggesting that further control of in-gel collagen self-assembly is required to elaborate fully functional biomaterials.

Mots clés: hydrogels; fibrin; collagen

Domaines: Polymères; Biomatériaux

Kun Wang, Marie Camman, Gervaise Mosser, Bernard Haye, Léa Trichet, et al.. Synthesis of Fibrin-Type I Collagen Biomaterials via an Acidic Gel. Molecules, 2022, 27 (7), pp.2099. ⟨10.3390/molecules27072099⟩. ⟨hal-03621606⟩