Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas (1) , Pierre Mahou (1) , Sophie Escot (1) , Chiara Stringari (1) , Nicolas B David (1) , Laure Bally-Cuif (2) , Nicolas Dray (2) , Michel Négrerie (1) , Willy Supatto (1) , Emmanuel Beaurepaire (1)

1 LOB - Laboratoire d'Optique et Biosciences
2 Neurogénétique du Poisson zébré / Zebrafish Neurogenetics

We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

Domaines: Optique / photonique; Sciences du Vivant [q-bio]

Júlia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas B David, et al.. Color TSFG microscopy of red blood cells and oxygenation. Advances in Microscopic Imaging, Proceedings of SPIE, the International Society for Optical Engineering, 12630, SPIE, pp.1, 2023, 9781510664692. ⟨10.1117/12.2670229⟩. ⟨hal-04372956⟩